STUDY OF THE EFFECT OF RABBIT IMMUNIZATION

The authors of the presented article set out to study the effect of immunization of rabbits with different types of yeast on the general antimicrobial activity of serum and the specific activity of its AMP fraction. The experimental group of rabbits consisted of 7 healthy chinchilla females weighing 1.5 kg, obtained from the nursery "LARSik" Kazakhstan, branch "Andreevka". The control group consisted of 4 of the same rabbits. The experiments were carried out in accordance with the interstate standard for the maintenance and care of laboratory animals (GOST 33217-2014). Rabbits were immunized with yeast cells - Candida albicans, Rhodotorula mu cilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cuta neum, Saccharomyces cerevisiae. AMP fractions were obtained by filtration of sera through filters with a pore diameter of 100 kDa. The total activity of the sera and their AMP fractions was assessed by the spectrophotometric method.
It was found that serum albumin at physiological concentrations (about 50 mg / ml and higher) has a direct antimicrobial effect on yeast and bacterial cells, while exhibiting a dose-dependent effect [10]. Determination of the albumin concentration in the present study showed no significant differences in these parameters in immunized and non-immunized animals. However, for some yeast species, a correlation was observed between the albumin concentration and the total serum activity.

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