DETERMINATION OF PHARMACEUTICAL-TECHNOLOGICAL PARAMETERS OF THE PLANT RAW MATERIAL EUPHORBIA HUMIFUSA WILLD.

Today, the development of pharmaceutical substances based on biologically active compounds from plants is highly relevant. In this context, it is of great importance to explore methods for the optimal extraction of biologically active substances from the raw material of the plant Euphorbia humifusa Willd., which grows in our country and is rich in biologically active compounds. Obtaining an extract that meets pharmacopoeial requirements, as well as determining the technological parameters of the plant raw material, is crucial for the research.

This scientific article presents the results of the pharmaceutical and technological parameters of the raw material from the plant Euphorbia humifusa Willd.

The purpose of this study. To determine the pharmaceutical and technological parameters of the aerial part of the plant Euphorbia humifusa Willd.

Methods and Materials. The research material consisted of the leaves and stems of Euphorbia humifusa Willd. Pharmaceutical and technological parameters of the plant raw material were determined, including specific gravity (dу, g/cm³), bulk density (d₀, g/cm³), tapped density (dH, g/cm³), porosity of the raw material, voidage of the raw material, free volume of the raw material layer (V), and the absorption coefficient of the extractant (Х, ml/g).

Results. The following parameters of the plant raw material were determined during the study: specific gravity (dу) – 1.31±0.017 g/cm³, bulk density (d₀) – 0.32±0.010 g/cm³, tapped density (dH) – 0.12±0.002 g/cm³, voidage – 0.75±0.014 g/cm³, porosity of the raw material  – 0.60±0.005 g/cm³, and free volume of the raw material layer – 0.90±0.011 g/cm³. Additionally, pharmacopeial quality indicators of the plant raw material were established: moisture content, 7.95±0.05%; total ash, 6.63±0.12%; and ash insoluble in a 10% HCl solution, 1.25±0.10%. The contents of heavy metals, such as cadmium, mercury, lead, and arsenic, and radionuclides, such as strontium-90 and cesium-137, in Euphorbia humifusa Willd. were within permissible limits.

Conclusion. Cadmium and mercury heavy metals were not detected in the composition of Euphorbia humifusa Willd., while the levels of lead and arsenic comply with the standards established in Pharmacopoeial Article 1.5.3.009 “Determination of Heavy Metals in Medicinal Plant Raw Materials and Herbal Preparations.”

1. Madeleine, E.; Olwen, M.; Grace, C.; Haris, S.L.; Niclas, N.; Henrik, T.; Nina, R. Global medicinal uses of Euphorbia L. (Euphorbiaceae). J. Ethnopharmacol. 2015, 76, 90–101.

2. Webster, G.L. Classification of the Euphorbiaceae. Ann. Mol. Bot. Gard. 1994, 81, 3–32.

3. Kemboi, D.; Xolani, P.; Moses, L.; Jacqueline, T. A review of the ethnomedicinal uses, biological activities and triterpenoids of Euphorbia species. Molecules 2020, 25, 4019.

4. Muhammad, A.; Abdul, S.; Muhammad, J.; Farhat, U.; Muhammad, O.; Ikram, U.; Jawad, A.; Muhammad, S. Flavonoids as prospective neuroprotectants and their therapeutic propensity in aging associated neurological disorders. Front. Aging Neurosci. 2019, 1–20.

5. Nabavi, S.M.; Samec, D.; Tomczyk, M.; Milella, L.; Russo, D.; Habtemariam, S.; Suntar, I.; Rastrelli, L.; Daglia, M.; Xiao, J. Flavonoid biosynthetic pathways in plants: Versatile targets for metabolic engineering. Biotechnol. Adv. 2018.

6. Suina I.O. Study of technological parameters and numerical quality indicators of raw materials Aristolochia clematitis L. // Suina I.O., Terninko I.I. - Drug development and registration. - 2017. - №4 (21). - С. 202-205

7. The State Pharmacopoeia of the Republic of Kazakhstan. – Almaty: Publishing house "Zhibek Zholy", 2008. - Vol.1. – p. 567.